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Therefore, over recent years prostate cancer biotherapy methods have been developed. Clinical trials with immune active agents reported in the last years have demonstrated efficacy in the treatment of prostate cancer. These agents include immune modulators, antibody targeting prostate-specific membrane antigen, and vaccines such as those targeting prostatic acid phosphatase, prostate-specific antigen, and cellular vaccines expressing granulocyte-macrophage colony stimulating factor [ 4 — 6 ].
Results from several recent clinical trials have suggested that immune-based therapies have clinical benefit in patients with prostate cancer with potentially less toxicity than traditional treatments [ 7 — 9 ]. Immunotherapy mediated by cytotoxic lymphocytes and lymphokine-activated lymphocytes LAKs was shown to be powerful in the destruction of drug-resistant tumor cells. These lymphocytes kill target cells by two main mechanisms, namely the perforin pathway and the Fas-ligand Fas-L pathway.
This improvement implies the development and the introduction of mononuclear leukocytes MLs activated by exogenous cytokines , or new methods of their cultivation. For this purpose liposomal and pegylated drug forms of interleukin-2 have been synthesized [ 11 , 12 ]. Cyclodextrins, natural or synthetic polysaccharides , as nanocarriers for bioactive compounds offer new opportunities [ 13 — 15 ].
ML was extracted from heparin stabilized periphery blood of healthy donor at ficoll-urografin gradient 1. The suspension of ML 9.
The suspension of ML 4. NK activity was determined towards NK-sensitive human erythroleukemia cell line K Cells were controlled and documented by Axiovert Zess, Germany. MLs were incubated with monoclonal antibodies specific for human antigens CD.
All statistical calculations were made using the software package Statistica 6. On the contrary IL-2 50 cells had not organized such clusters Figure 4. MTT added. Induction of new cell populations of activated peripheral blood mononuclear cells versus control SSC-H:site scatter height, FSC-H:forward scatter height. At present a therapy of hormone-resistant prostate cancer possesses very limited resources. In addition, modern methods of chemotherapy and X-ray therapy often result in side effects, including immunosuppression and thus impair patient's life quality.
The undermining of immunological and antitumor status as a result of the treating may lead to the disease progression. Therefore, it seems to be of current interest to develop new methods of biotherapy of hormone-resistant prostate cancer.
At present different types of dendritic-cells-based vaccine, are undergoing clinical trials [ 17 , 18 ]. It is well-known that macrophages and especially dendritic cells DCs secrete cytokines, particularly IL, thus improving IL-2 effect. The main disadvantage of the current adoptive immunotherapy methods is the limited target effect produced upon one of the mentioned immune system components.
In particular, DC vaccines action is aimed to the creation of specific immune response to the tumor antigen. However, as tumor is heterogenic, a part of the cells population does not express cancer-associated antigens and MHC molecules enabling so the neoplastic cells to escape an immune response effect.
Usually immune clinical cancer treatment involves the use of high cytokine dose, reaching in the case of IL-2 dose near or superior to IU. This dose creates serious side effects [ 22 ]. If you have a question in relation to how we process your personal data you can contact our Data Protection Officer via email info abcloudgroup. Personal Data means information that can directly or indirectly identify you "Personal Data". This typically includes information such as your name, address, email address, and telephone number, but can also include other information such as IP address, shopping habits, and information about your lifestyle or preferences such as your hobbies and interests.
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